pET-41a(+)大肠杆菌表达载体说明

编号\r  

北京华越洋生物VECT4930\r  

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pET41a载体基本信息\r  别名:\r  

质粒类型:\r  表达水平:\r  克隆方法:\r  载体大小:\r  5'\r  测序引物:\r  3'\r  测序引物:\r  载体标签:\r  载体抗性:\r  备注:\r  

载体名称\r  

pET-­‐41a(+)\r  

稳定性:\r  组成型:\r  

病毒/非病毒:\r  \r  

pET41a载体质粒图谱和多克隆位点信息\r  

pET41a,\r  pET\r  41a\r  大肠杆菌蛋白表达\r  高\r  

多克隆位点，性内切酶\r  5933\r  bp\r  

pGEX-­‐5':\r  5'-­‐GGGCTGGCAAGCCACGTTTGGTG-­‐3'\r  T7t:\r  5'-­‐GCTAGTTATTGCTCAGCGG-­‐3'\r  N-­‐GST,\r  N-­‐His,\r  N-­‐Thrombin,\r  N-­‐EK,\r  N-­‐S\r  卡那\r  

载体带有GST蛋白纯化标签，pET41\r  a,b,c\r  的差异是在于多克隆位点。\r  瞬时表达\r  组成型\r  非病毒\r  

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pET41a载体简介\r  

pET41系列载体是设计用来克隆和高水平表达蛋白质的载体，载体融合表达一个含有220个氨基酸的GST标签蛋白纯化片段。pET41a载体的单一的多克隆位点见上面的环状质粒图谱。注意：载体序列是以pBR322质粒的编码规矩进行编码的，所以T7蛋白表达区在质粒图谱上面是反向的。\r  \r  

T7\r  RNA聚合酶启动的克隆和表达区域在质粒图谱中也被标注了出来。质粒的F1复制子是被定向的，所以在T7噬菌体聚合酶的作用下，包含有蛋白编码序列的病毒\r  粒子能够产生，并启动蛋白表达，同时蛋白表达将被T7终止子序列(Cat.\r  No.\r  69337-­‐3)的作用下终止蛋白翻译。\r  \r  

pET-­‐41a载体上面含有一个EK蛋白酶切位点，当将目的基因使用载体上面的PshAI性内切酶位点插入进去时，可以通过EK蛋白酶将载体上的融合的所有氨基酸序列包括GST标签序列，全部切除掉。\r  

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pET41a载体序列\r  

ORIGIN

1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA 61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT 121 TGTTTAGCAG CCTAGGTATT AATCAATTAG TGGTGGTGGT GGTGGTGGTG GTGCTCGAGT 181 GCGGCCGCAA GCTTGTCGAC GGAGCTCGCC TGCAGGCGCG CCAAGGCCTG TACAGAATTC 241 GGATCCCCGA TATCCCATGG GACTCTTGTC GTCGTCATCA CCGGAGCCAC CACCGGTACC 301 CAGATCTGGG CTGTCCATGT GCTGGCGTTC GAATTTAGCA GCAGCGGTTT CTTTCATACC 361 AATTGCAGTA CTACCGCGTG GCACCAGACC CGCGGAGTGA TGGTGATGGT GATGACCAGA 421 ACCACTAGTT GAACCATCCG ATTTTGGAGG ATGGTCGCCA CCACCAAACG TGGCTTGCCA 481 GCCCTGCAAA GGCCATGCTA TATACTTGCT GGATTTCAAG TACTTATCAA TTTGTGGGAT 1 AGCTTCAATA CGTTTTTTAA AACAAACTAA TTTTGGGAAC GCATCCAGGC ACATTGGGTC 601 CATGTATAAA ACAACATCAA GAGCGTCATA CAACATGAAG TCAGGATGGG TTACATGATC 661 ACCATTTAAA TATGTTTTAT GACATAAACG ATCTTCGAAC ATTTTCAGCA TTTCAGGTAG 721 CTTGCTAAGA AAATCAACTT TGAGAGTTTC AAAGTCTTTA CTATATGCAA TTCTCGAAAC 781 ACCGTATCTA ATATCCAAAA CCGCTCCTTC AAGCATTGAA ATCTCTGCAC GCTCTTTTGG 841 ACAACCACCC AACATGTTGT GCTTGTCAGC TATATAACGT ATGATGGCCA TAGACTGTGT 901 TAATTTAACA TCACCATCAA TATAATAAGG AAGATTGGGA AACTCCAAAC CCAATTCAAA 961 CTTTTTGTTT CGCCATTTAT CACCTTCATC GCGCTCATAC AAATGCTCTT CATATTTTTC 1021 TTCAAGATAT TCCAAAAGAA GTCGAGTGGG TTGCACAAGG CCCTTAATTT TCCAATAACC 1081 TAGTATAGGG GACATATGTA TATCTCCTTC TTAAAGTTAA ACAAAATTAT TTCTAGAGGG 1141 GAATTGTTAT CCGCTCACAA TTCCCCTATA GTGAGTCGTA TTAATTTCGC GGGATCGAGA 1201 TCGATCTCGA TCCTCTACGC CGGACGCATC GTGGCCGGCA TCACCGGCGC CACAGGTGCG 1261 GTTGCTGGCG CCTATATCGC CGACATCACC GATGGGGAAG ATCGGGCTCG CCACTTCGGG 1321 CTCATGAGCG CTTGTTTCGG CGTGGGTATG GTGGCAGGCC CCGTGGCCGG GGGACTGTTG 1381 GGCGCCATCT CCTTGCATGC ACCATTCCTT GCGGCGGCGG TGCTCAACGG CCTCAACCTA 1441 CTACTGGGCT GCTTCCTAAT GCAGGAGTCG CATAAGGGAG AGCGTCGAGA TCCCGGACAC 1501 CATCGAATGG CGCAAAACCT TTCGCGGTAT GGCATGATAG CGCCCGGAAG AGAGTCAATT 1561 CAGGGTGGTG AATGTGAAAC CAGTAACGTT ATACGATGTC GCAGAGTATG CCGGTGTCTC 1621 TTATCAGACC GTTTCCCGCG TGGTGAACCA GGCCAGCCAC GTTTCTGCGA AAACGCGGGA 1681 AAAAGTGGAA GCGGCGATGG CGGAGCTGAA TTACATTCCC AACCGCGTGG CACAACAACT 1741 GGCGGGCAAA CAGTCGTTGC TGATTGGCGT TGCCACCTCC AGTCTGGCCC TGCACGCGCC 1801 GTCGCAAATT GTCGCGGCGA TTAAATCTCG CGCCGATCAA CTGGGTGCCA GCGTGGTGGT 1861 GTCGATGGTA GAACGAAGCG GCGTCGAAGC CTGTAAAGCG GCGGTGCACA ATCTTCTCGC 1921 GCAACGCGTC AGTGGGCTGA TCATTAACTA TCCGCTGGAT GACCAGGATG CCATTGCTGT 1981 GGAAGCTGCC TGCACTAATG TTCCGGCGTT ATTTCTTGAT GTCTCTGACC AGACACCCAT 2041 CAACAGTATT ATTTTCTCCC ATGAAGACGG TACGCGACTG GGCGTGGAGC ATCTGGTCGC 2101 ATTGGGTCAC CAGCAAATCG CGCTGTTAGC GGGCCCATTA AGTTCTGTCT CGGCGCGTCT 2161 GCGTCTGGCT GGCTGGCATA AATATCTCAC TCGCAATCAA ATTCAGCCGA TAGCGGAACG 2221 GGAAGGCGAC TGGAGTGCCA TGTCCGGTTT TCAACAAACC ATGCAAATGC TGAATGAGGG 2281 CATCGTTCCC ACTGCGATGC TGGTTGCCAA CGATCAGATG GCGCTGGGCG CAATGCGCGC 2341 CATTACCGAG TCCGGGCTGC GCGTTGGTGC GGACATCTCG GTAGTGGGAT ACGACGATAC 2401 CGAAGACAGC TCATGTTATA TCCCGCCGTT AACCACCATC AAACAGGATT TTCGCCTGCT

2461 GGGGCAAACC AGCGTGGACC GCTTGCTGCA ACTCTCTCAG GGCCAGGCGG TGAAGGGCAA 2521 TCAGCTGTTG CCCGTCTCAC TGGTGAAAAG AAAAACCACC CTGGCGCCCA ATACGCAAAC 2581 CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT 21 GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTAAGTTA GCTCACTCAT TAGGCACCGG 2701 GATCTCGACC GATGCCCTTG AGAGCCTTCA ACCCAGTCAG CTCCTTCCGG TGGGCGCGGG 2761 GCATGACTAG CATGATCGTG CTCCTGTCGT TGAGGACCCG GCTAGGCTGG CGGGGTTGCC 2821 TTACTGGTTA GCAGAATGAA TCACCGATAC GCGAGCGAAC GTGAAGCGAC TGCTGCTGCA 2881 AAACGTCTGC GACCTGAGCA ACAACATGAA TGGTCTTCGG TTTCCGTGTT TCGTAAAGTC 2941 TGGAAACGCG GAAGTCAGCG CCCTGCACCA TTATGTTCCG GATCTGCATC GCAGGATGCT 3001 GCTGGCTACC CTGTGGAACA CCTACATCTG TATTAACGAA GCGCTGGCAT TGACCCTGAG 3061 TGATTTTTCT CTGGTCCCGC CGCATCCATA CCGCCAGTTG TTTACCCTCA CAACGTTCCA 3121 GTAACCGGGC ATGTTCATCA TCAGTAACCC GTATCGTGAG CATCCTCTCT CGTTTCATCG 3181 GTATCATTAC CCCCATGAAC AGAAATCCCC CTTACACGGA GGCATCAGTG ACCAAACAGG 3241 AAAAAACCGC CCTTAACATG GCCCGCTTTA TCAGAAGCCA GACATTAACG CTTCTGGAGA 3301 AACTCAACGA GCTGGACGCG GATGAACAGG CAGACATCTG TGAATCGCTT CACGACCACG 3361 CTGATGAGCT TTACCGCAGC TGCCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC 3421 ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG 3481 CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG CGCAGCCATG ACCCAGTCAC 31 GTAGCGATAG CGGAGTGTAT ACTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG 3601 AGTGCACCAT ATATGCGGTG TGAAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC 3661 AGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA 3721 GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA 3781 GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG 3841 CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT 3901 CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC 3961 CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT 4021 TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC 4081 GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA 4141 TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA 4201 GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG 4261 TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG 4321 CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT 4381 AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA 4441 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG 4501 ATTTTGGTCA TGAACAATAA AACTGTCTGC TTACATAAAC AGTAATACAA GGGGTGTTAT 4561 GAGCCATATT CAACGGGAAA CGTCTTGCTC TAGGCCGCGA TTAAATTCCA ACATGGATGC 4621 TGATTTATAT GGGTATAAAT GGGCTCGCGA TAATGTCGGG CAATCAGGTG CGACAATCTA 4681 TCGATTGTAT GGGAAGCCCG ATGCGCCAGA GTTGTTTCTG AAACATGGCA AAGGTAGCGT 4741 TGCCAATGAT GTTACAGATG AGATGGTCAG ACTAAACTGG CTGACGGAAT TTATGCCTCT 4801 TCCGACCATC AAGCATTTTA TCCGTACTCC TGATGATGCA TGGTTACTCA CCACTGCGAT 4861 CCCCGGGAAA ACAGCATTCC AGGTATTAGA AGAATATCCT GATTCAGGTG AAAATATTGT 4921 TGATGCGCTG GCAGTGTTCC TGCGCCGGTT GCATTCGATT CCTGTTTGTA ATTGTCCTTT 4981 TAACAGCGAT CGCGTATTTC GTCTCGCTCA GGCGCAATCA CGAATGAATA ACGGTTTGGT 5041 TGATGCGAGT GATTTTGATG ACGAGCGTAA TGGCTGGCCT GTTGAACAAG TCTGGAAAGA

5101 AATGCATAAA CTTTTGCCAT TCTCACCGGA TTCAGTCGTC ACTCATGGTG ATTTCTCACT 5161 TGATAACCTT ATTTTTGACG AGGGGAAATT AATAGGTTGT ATTGATGTTG GACGAGTCGG 5221 AATCGCAGAC CGATACCAGG ATCTTGCCAT CCTATGGAAC TGCCTCGGTG AGTTTTCTCC 5281 TTCATTACAG AAACGGCTTT TTCAAAAATA TGGTATTGAT AATCCTGATA TGAATAAATT 5341 GCAGTTTCAT TTGATGCTCG ATGAGTTTTT CTAAGAATTA ATTCATGAGC GGATACATAT 01 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC 61 CACCTGAAAT TGTAAACGTT AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA 5521 GCTCATTTTT TAACCAATAG GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGA 5581 CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA AAGAACGTGG 51 ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA TGGCCCACTA CGTGAACCAT 5701 CACCCTAATC AAGTTTTTTG GGGTCGAGGT GCCGTAAAGC ACTAAATCGG AACCCTAAAG 5761 GGAGCCCCCG ATTTAGAGCT TGACGGGGAA AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA 5821 AGAAAGCGAA AGGAGCGGGC GCTAGGGCGC TGGCAAGTGT AGCGGTCACG CTGCGCGTAA 5881 CCACCACACC CGCCGCGCTT AATGCGCCGC TACAGGGCGC GTCCCATTCG CCA //

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其他大肠杆菌表达载体：

pBV221

pDsRed-Express2 pCold TF pCold I

pBAD202/D-TOPO pBad/Myc-His B pBad/His A pET-23c(+) pET-12a(+) pBad24 pQE-32 pQE-60 pET-50b(+) pET-26b(+) pET-14b pET-20b(+) pET-21a(+) pET-27b(+) pET-43.1c(+) pET-44c(+) pTrcHis2 B pRSET-CFP pET300/NT-DEST pGEX-4T-3 pG-KJE8

ptdTomato pBV220 pCold IV pE-SUMO pACYC184 pBAD-TOPO pET-23(+) pET-11b(+) pQE-82L pQE-9 pQE-70 pET-49b(+) pET-32a(+) pET-16b pET-21d(+) pET-24a(+) pET-28a(+) pET-43.1b(+) pET-46 EK/LIC pTrcHis2 A pRSET-EmGFP pGEX-5X-2 pGro7

pET-52b(+) pCold-GST pCold III pCold-ProS2 pBAD/Thio-TOPO pET-23b(+) pET-12b(+) pET-11a(+) pQE-81L pQE-16 pQE-40 pET-48b(+) pET-21b(+) pET-15b pET-21c(+) pET-24d(+) pET-30a(+) pET-43.1a(+) pET-37b(+) pET303/CT-His pRSET-BFP

pBlueScript SK(+) pET-SUMO

pAmCyan pColdS-SUMO pCold II pBAD102/D-TOPO pBad/Myc-His C pBad/His B pET-23a(+) pET-12c(+) pET-11c(+) pQE-80L pQE-31 pET-51b(+) pET-47b(+) pET-22b(+) pET-19b pET-21b(+) pET-25b(+) pET-42a(+) pET-44a(+) pTrcHis2 C pET302/NT-His pGFPuv pBad43 pG-Tf2 pSE380

pBad/Myc-His A pBad/His C

pET301/CT-DEST pGEM-T

pET-17b pTf16 pSUMO pKD3 pTYB2 pET102/D-TOPO pET-28c(+) pProEX HTc pKD13 pTWIN2 pCDFDuet-1 pProEX HTb pKD46

pMAL-p5x pProEX HTa pTYB1 pBluescript II SK(+) pET-30b(+)

pBluescript II KS(-) pTYB12

pMAL-p5e PinPoint Xa-3 pSP pET-5b(+) pET-5a(+) pkk232-8 pMAL-c5x

pET-43.1 EK/LIC pET-31b(+) pGEX-4T-2 pET-28b(+) pBR322 pEcoli-6xHN-GFPuv pGEX-2T pTrcHis A pGEX-6P-3 pGEX-5X-1 pMal-c2E pET-45b(+) pET-40b(+) pET-32 Xa/LIC pET-30 EK/LIC pET-29a(+) pET-23d(+)

pACYCDuet-1 PinPoint Xa-2 pTWIN1 pBad/gIII C pMal-p4X pCYB1 pMal-p2E pET-41 EK/LIC pET-3b(+) pETDuet-1 pET-His pKD4 pKJE7

pRSFDuet-1 pET-41b(+) pGEX-6P-2 pGEX-2TK pMal-c2X pET-44b(+) pET-33b(+) pET-32c(+) pET-30c(+) pET-24c(+) pET-11d(+)

pEGM-11ZF(+) PinPoint Xa-1 pTYB11 pBad/gIII B pMal-p2G pEZZ18 pMal-p2X pMal-c4X pET-41a(+) pGEX-4T-1 pALEX a,b,c pKD20 pRSET B pCOLADuet-1 pET-42b(+) pGEX-6P-1 pRSET A pRSET C pET-42c(+) pET-39b(+) pET-32b(+) pET-29c(+) pET-24b(+) pBad33

pEGM-7ZF(+) pSP73 pTXB1 pBad/gIII A pkk223-3 pBAD18

pET-44 EK/LIC pTrcHis B pGEX-3X pTrc99a pACYC177 pMXB10 pGEX-KG pTrcHis C pET-3a(+) pGEX-5X-3 pMal-c2G pQE-30 pET-41c(+) pET-32 EK/LIC pET-30 Xa/LIC pET-29b(+) pET-24(+)

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