专利名称：Chromogenic test in the presence of cells发明人：WATZELE, MANFRED,NIKOLAUS,
THOMAS,RODE, HANS-JUERGEN
申请号：EP09015830.4申请日：20051212公开号：EP2177622B1公开日：20130116
摘要：Colorimetric measurement of an enzyme reaction, comprises providing a cellsuspension or cell component containing cell lyzate, adding a chromogenic substrate tostart the enzyme reaction, adding hydrochloride as a first further substance to terminatethe enzyme reaction, and adding a second further substance after terminating theenzyme reaction that dissolves the cell membrane. An independent claim is included for akit for colorimetric measurement of enzyme reactions in cell suspensions or cell
component containing cell lyzates, comprising hydrochloride as a chromogenic substrate,a first substance to terminate the enzyme reaction, and Triton X 100 (RTM: Non-ionicsurfactant) as a second substance that dissolves when added to the cell membrane,where the second substance equalizes the refractive index of the suspension or the celllyzates of the cells or cell components.
申请人：ROCHE DIAGNOSTICS GMBH,ROCHE DIAGNOSTICS GMBH,ROCHEDIAGNOSTICS GMBH,F. HOFFMANN-LA ROCHE AG,HOFFMANN LA ROCHE,F.HOFFMANN-LA ROCHE AG
地址：DE,CH
国籍：DE,CH
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