SCREENING AND SORTING OF SINGLE CELLS

专利名称：SCREENING AND SORTING OF SINGLE CELLS发明人：LAMMERTYN, Jeroen,VANHOORELBEKE,

Karen,GEUKENS, Nick,HORTA,Sara,BREUKERS, Jolien

申请号：EP19802139.6申请日：20191108公开号：EP3877085A1公开日：20210915

摘要：In general the present invention concerns 1) single cell trapping of a viable cellin separate well from a plurality of wells in an array of wells, 2) single cell analysis for theselected cell and 3) single cell lifting of the yet viable cell from the well by an opticaltweezer. Furthermore resent invention concerns a cell trap and lift device for B

lymphocytes, the device comprising an array of wells in in polymer matrix comprising anoff-stoichiometry thiol-ene polymer of the group consisting of off- stoichiometry thiol-ene (OSTE) and off- stoichiometry thiol-ene-epoxy (OSTE+) or a combination thereof thathave been grafted with methacrylated polyethylene glycol (methoxy polyethylene glycolmethacrylate or (M-PEG-M)) of a number average molecular weight of Mn 2000. Itfurthermore concerns using the B lymphocyte trap and lift device for trapping single Blymphocyte cells in wells of the device of present invention and lifting said cell from thecell trapping well by optical tweezers, preferably single beam tweezers.

申请人：Katholieke Universiteit Leuven

地址：KU Leuven Research & Development Waaistraat 6 Box 5105 3000 Leuven BE

国籍：BE

代理机构：Beck, Michaël Andries T.

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