Science&TechnologyVision科技视界超声辅助醇提桑枝中1-脱氧野尻霉素王中兴渊安康学院现代农业与生物科技学院袁陕西安康725000冤揖摘要铱1-脱氧野尻霉素渊DNJ冤是一种哌啶生物碱袁具有降血糖尧抗病毒等作用袁在医疗保健方面具有重要的研究价值遥以乙醇为溶剂袁结合超声处理提取桑枝中的DNJ袁研究料液比尧乙醇浓度和超声时间对DNJ提取的影响袁结果表明院桑枝中DNJ在料液比为1:20袁乙醇浓度为65%袁超声处理时间为35min时提取含量最高袁达到0.253%遥揖关键词铱桑枝曰1-脱氧野尻霉素曰超声波曰高效液相色谱中图分类号院TQ461文献标识码院ADOI院10.19694/j.cnki.issn2095-2457.2019.35.123文章编号院2095-2457渊2019冤35-0252-003TheExtractionof1-DeoxynojirimycinbyEthanolUsingUltrasonicAssistantfromRamulusMoriWANGZhong-xing(SchoolofModernAgriculture&Biotechnology袁AnKanguniversity,AnkangShaanxi725000,China)揖Abstract铱1-Deoxynojirmycin(DNJ)whichisknownashydroxylalkaloidhashypoglycemicandantiviraleffects,andhasimportantresearchvalueinhealthcare.TheeffectforDNJextractionwhichincludessolid-liquidratio,ethanolconcentrationandultrasonictimewasstudied.TheresultsshowthattheDNJextractionisthehighestwhenliquidmaterialratiois1:20,ethanolconcentrationis65%,ultrasonicextractiontimeis35min,andisupto0.253%.揖Keywords铱1-deoxynojirimycin;Ultrasonicassistedextraction;Highperformanceliquidchromatography1-渊1-deoxynojirimycin,DNJ冤袁C6H13NO4袁163.7[1]袁[2-3]琢-袁尧尧[4-6]袁[7-8]袁[9]袁[10]袁[11]袁袁[12-14]袁遥DNJ尧[15-17]袁DNJ袁袁DNJ遥DNJ袁袁袁袁DNJ袁袁遥1.3.1袁袁70益袁遥1.3.2渊1冤遥51g,550ml袁55%10ml尧20ml尧30ml尧40ml50ml袁30min袁10min袁50Hz袁10s袁袁12000r/min袁15min袁遥渊2冤遥51g袁550ml袁20ml袁55%尧65%尧75%尧85%95%袁30min,袁10min,50Hz袁10s袁袁12000r/min袁15min袁遥渊3冤遥51g袁550ml袁55%20ml袁30min袁袁10min尧20min尧30min尧40min50min袁50Hz袁10s袁袁12000r/min袁15min袁11.1实验材料与方法曰遥冤尧冤尧院2院DNJ渊渊袁1.21260Infinity渊KQ-250D渊FW-100D渊冤袁DGX-914313-1渊冤遥1.3冤袁冤袁作者简介院王中兴渊1981要冤袁男袁吉林吉林人袁任教于安康学院袁硕士袁讲师袁研究方向为微生物学遥淫基金项目院陕西省科技厅项目渊2013K01-26冤曰安康学院校级项目渊2013AYPYZR05冤遥252科技视界Science&TechnologyVisionScience&TechnologyVision科技视界遥1.3.3袁尧袁遥1.4DNJ要[18]2结果袁分析2袁遥2.1DNJ袁y=10255x+133.16袁0.9903遥图1DNJ标准曲线2.2图2标准品色谱图2.3图3样品色谱图2.44袁1g:20ml袁DNJ遥图4不同料液比对DNJ提取的影响2.5图5不同乙醇浓度对DNJ提取的影响5袁65%袁遥2.6图6不同超声时间下DNJ含量柱形图6袁30min袁遥2.7袁遥表1正交因素水平表A渊%冤BC渊min冤1601院15252651院20303701院2535Science&TechnologyVision科技视界253Science&TechnologyVision科技视界表2直观分析表ABCD渊冤DNJ渊%冤12111312210.413320.11452330.117621130.135722210.162833312220.2413230.1820.157910.12230.14430.17210.15310.22110320.1950.1930.1270.15230.1600.1400.1780.17310.0730.0530.0510.021表3方差分析表FF0.00821.6844.4600.00521.0534.4600.00521.0534.4600.0282尧3袁DNJ院65>35minA2B2C3袁%=尧1遥:20DNJ尧2.8遥DNJ袁DNJ0.253%袁袁遥3结论袁65%袁1:20袁35min袁袁0.253%遥咱1暂,揖参.考1-文献铱咱linked2[暂JSaunier].,B,Kilker,2012((DNJ)R03)J:,44Tkacz-48.JS,etal.aninhibitorcomplexofprocessingoligosaccharideglucosidasesformation[J].byJBiol1-InhibitiondeoxynojirimycinofN-Chem,1982,257,2科技视界Science&TechnologyVision(咱23inhibits3暂):Li14155Y-G14161.,JiDF,ZhongS,etal.1-deoxynojirimycinstreptozotocinglucose咱of4暂-inducedabsorptiondiabeticandmiceaccelerates[J].SciglucoseRep,2013metabolism,3:1377.inMice1Hu-DeoxynojirimycinXQ,ThakurfromK,ChenMulberryGHLeaves,etal.onMetabolicdb/EffectMetabolomicsUsing咱derived5暂Sevsek[AJLiquidChromatography-MassSpectrometrydbDiabeticBased,].JAgricFoodChem,2017.glucocerebrosidasefrom1-CelanM,ErjavecB,etal.Bicyclicisoureas咱16暂KwonHJ,[deoxynojirimycinJChung].Orgarepotentinhibitorsofbeta-JBiomolY,KimChemJ,2016,14(37):8670-8673.emphasis-deoxynojirimycinandaqueousmulberryY,etal.leafComparisonofvitroonpostprandialhypoglycemiceffects:inextractvivoandwithin咱Liver7暂LiustudiesQ,[JLi].JAgricFoodChem,2011,59(7):3014-3019.Mice咱deoxynojirimycin8暂Do[JInjuryX,LiC,etal.1-DeoxynojirimycinAlleviates].MoleculesandImprovesHepaticGlucoseMetabolismindb/dbHJ,,Chung2016,21J(3)H:279.,HwangJW,etlipid[咱JInsulin9].暂FoodmetabolismisolatedandmitochondrialfromBacillusfunctionsubtilisinhighimprovesal.-fat-fedhepatic1-miceLiuQChem,LiToxicolX,Li,2015C,et,75al.:1-7.1-PathwayResistance2015inSkeletalviaActivationMuscleofofdbInsulinDeoxynojirimycin/dbSignalingMice[J].PI3KAlleviatesMolecules/AKT,咱attenuates10暂,20E(S12,):Kijima21700-R21714.,HonmaT,etal.1-umbilical咱veinhighendothelialglucosecells-accelerated[J].ExpGerontolsenescenceDeoxynojirimycin,2014,55in:63human-69.mulberry11暂TsudukiT,KikuchiI,KimuraT,etthrough2013increases1-deoxynojirimycinpreventsdiet-inducedal.Intakeobesityof咱pentafluorobenzyl12暂,139inadiponectininmice[J].FoodChem,Liu(1M-,4Wang):16-23.S,ZhouYD,etal.Animinosugarimmunosuppressant-1Bioorgfor-deoxynojirimycinasanovelpotentialN-咱13暂MedChem,Lett,,the2012treatment,,22(.1):5of-Th2570.-relateddiseases[J].咱14暂,,[,J]..,20071-(01):22-24.2010[J].咱15暂(01):109,-114.,,咱16暂,[J].,.261-,,2010(03):558-563.咱17暂(DNJ),,,[J.].Bacillussubtilis,2010B2(17):1290--293.677.1-.[J].,2007(04):674-咱18暂,146-149.1-,,.[J].,2006-(01):
