专利名称：MEMBRANE SCAFFOLD PROTEINS发明人：SLIGAR, Stephen, G.,BAYBURT, Timothy, H.申请号：EP01995157.3申请日：20011120公开号：EP1345959B1公开日：20110525
摘要：Membrane proteins are difficult to express in recombinant form, purify, andcharacterize, at least in part due to their hydrophobic or partially hydrophobic
properties. Membrane scaffold proteins (MSP) assemble with target membrane or otherhydrophobic or partially hydrophobic proteins or membrane fragments to form solublenanoscale particles which preserve their native structure and function; they are improvedover liposomes and detergent micelles. In the presence of phospholipid, MSPs formnanoscopic phospholipid bilayer disks, with the MSP stabilizing the particle at theperimeter of the bilayer domain. The particle bilayer structure allows manipulation ofincorporated proteins in solution or on solid supports, including for use with suchsurface-sensitive techniques as scanning probe microscopy or surface plasmonresonance. The nanoscale particles facilitate pharmaceutical and biological research,structure/function correlation, structure determination, bioseparation, and drugdiscovery.
申请人：UNIV ILLINOIS
地址：US
国籍：US
代理机构：Fisher, Adrian John
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