Viral vectors for use in monitoring HIV drug resis

专利名称：Viral vectors for use in monitoring HIV drug

resistance

发明人：Jian-yun Dong申请号：US09559244申请日：20000426公开号：US06410013B1公开日：20020625

摘要：Recombinant expression vectors and methods are provided for detecting HIVand monitoring HIV drug resistance. The method comprises: taking a culture of cells;adding a recombinant viral vector into the culture to transduce the cells, the recombinantviral vector comprising a reporter sequence comprising a reporter gene whoseexpression is regulated by a protein specific to HIV viruses which is expressed from agenome of an HIV virus upon infection of a cell in the culture that is transduced by therecombinant viral vector, and a receptor sequence comprising CD 4 and one or morecoreceptor genes, expression of the coreceptor genes facilitating productive infection ofthe transduced cell and enabling HIV virus which has infected the transduced cell toreplicate and infect non-infected cells in the culture of the cells transduced by therecombinant viral vector; infecting the transduced cells with a sample containing HIV;adding one or more anti-HIV agents to the cell culture; and detecting a change in a levelof expression of the reporter gene in cells.

申请人：MUSC FOUNDATION FOR RESEARCH DEVELOPMENT

代理机构：Wilson Sonsini Goodrich & Rosati

代理人：David J. Weitz

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